recombinant human vascular endothelial growth factor (rhvegf)  (PeproTech)

Journal: STAR Protocols

Article Title: Protocol for differentiation of monocytes and macrophages from human induced pluripotent stem cells

doi: 10.1016/j.xpro.2024.103217

Figure Lengend Snippet:

Article Snippet: Recombinant human (rh) vascular endothelial growth factor (rhVEGF) , PeproTech , 100-20.

Techniques: Recombinant, Purification, Saline, Blocking Assay, Control, Generated, Software, Cell Culture, Dissection, Microscopy, Cell Counting, Confocal Microscopy, Flow Cytometry

Journal: Bio-Design and Manufacturing

Article Title: Biofabrication of nanocomposite-based scaffolds containing human bone extracellular matrix for the differentiation of skeletal stem and progenitor cells

doi: 10.1007/s42242-023-00265-z

Figure Lengend Snippet: Nanoclay-based inks support sustained release of VEGF in the CAM model. Macrographs during a sample implantation and b retrieval: (i) empty, (ii) AB, (iii) LAB, and VEGF-loaded (iv) AB and (v) LAB 3D-printed scaffolds. c Chalkley score of vascularized samples and controls. d – g Histological micrographs of samples stained for (i, ii) Goldner’s Trichrome and (iii, iv) Alcian Blue & Sirius Red. Statistical significance was assessed using one-way ANOVA. Data are presented as mean±standard deviation, n =4, * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001. Scale bars: a , b 10 mm, d – g 100 µm. VEGF: vascular endothelial growth factor; CAM: chick chorioallantoic membrane; AB: alginate-bone-ECM; LAB: Laponite-alginate-bone-ECM; ANOVA: analysis of variance

Article Snippet: Scaffolds with nanoclay and LAP-free were 3D printed, crosslinked following 10 min exposure to 100 mmol/L CaCl 2 and allowed to adsorb for 30 min with recombinant human vascular endothelial growth factor (rhVEGF 165, PeproTech, USA) at 100 μg/mL at 4 °C.

Techniques: Staining, Standard Deviation, Membrane

Journal: Acta biomaterialia

Article Title: Intravitreal injectable hydrogel rods with long-acting bevacizumab delivery to the retina.

doi: 10.1016/j.actbio.2023.09.025

Figure Lengend Snippet: Fig. 9. (a) Fundus and ultrasound images showing intravitreal stability and degradation of hydrogel rods. Images represent the state at the following periods: right after injection, d 1, d 4, d 8, d 14, d 30, d 60, d 90, and d 120. Concentration of proinflammatory factors ((b) rabbit TNF- α and (c) rabbit VEGF) in vitreous body injected with hydrogel rods. Rabbit eyes without any injections were used as controls. Data are presented as mean ± SD (Two rabbits, four eyes, n = 4). ∗P < 0.05; ns = no significant difference. The rabbit VEGF does not have binding specificity against BCZ.

Article Snippet: Recombinant human vascular endothelial growth factor rhVEGF) 165 protein, substrate reagents, and stop solution were urchased from R&D systems (Minneapolis, MN, USA).

Techniques: Injection, Concentration Assay, Binding Assay